Stream implementations can and do ignore backpressure; and some spec-defined features explicitly break backpressure. tee(), for instance, creates two branches from a single stream. If one branch reads faster than the other, data accumulates in an internal buffer with no limit. A fast consumer can cause unbounded memory growth while the slow consumer catches up — and there's no way to configure this or opt out beyond canceling the slower branch.
This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.
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